In our studies, There were three associated with HSA in freshly collected plasma from normal volunteers, HSA, HSA + cysteine, And HSA + glucose in the ratio parallel to50:25:25. We addressed this debate by using iodoacetamide to block the free thiol of HSA in fresh plasma, ceasing its reaction with plasma cysteine. When iodoacetamide was injected into a vacutaner tube as blood was compiled, The HSA was changed by iodoacetamide, With 20 30% give as the mixed disulfide with cysteine (HSA + cys).
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